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. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Nat Cell Biol. 2013 Apr 21;15(5):491–501. doi: 10.1038/ncb2720

Figure 1.

Figure 1

DAF-16/FOXO binds to the chromatin remodeller SWI/SNF. (a) C. elegans strains yielding inactive cytoplasmic DAF-16/FOXO (daf-18(mg198lf)/PTEN) or active nuclear DAF-16/FOXO (daf-2(e1370ts)/insulin-IGF receptor) were shifted for 20 h to restrictive temperature, lysed and lysates separated on a Superose 6 size-exclusion column. Fractions were analysed by SDS-PAGE and western blotting. (b) Schematics of the screen for DAF-16/FOXO cofactors. (c) SDS-PAGE/silver stain analysis of large-scale α-GFP immunoprecipitations from indicated strains. (d,e) Confirmatory co-IPs. DAF-16/FOXO::FLAG (d) or SWSN-4/BRG1::GFP (e) were immunoprecipitated from whole-worm lysates of the indicated strains. 50 U/ml Benzonase was added to eliminate DNA- or RNA-mediated interactions. Samples were analysed by SDS-PAGE and western blotting. For some inputs only fractions were loaded: 2% for SWSN-1::GFP (d), 2% for DAF-16::FLAG (e), and 20% for SWSN-4::GFP (e).