Skip to main content
. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Biochemistry. 2000 Sep 19;39(37):11514–11522. doi: 10.1021/bi0010468

Figure 4. Restriction and Purification of the Experimental Molecule.

Figure 4

Shown is an autoradiogram of a 5% non-denaturing gel. Two experiments are shown, one performed with a concentration of 500 nM DNA, and the other with a concentration of 100 nM DNA. The restriction is only partial, but streptavidin bead treatment removes incompletely restricted material. The extra strand is a control to ensure that no dissociation has occurred.

In both experiments, it is clear that the final molecule can be prepared cleanly, and that it does not dissociate under the conditions shown. The sequence of the extra strand is: 5′-CTAGACAGTA.TGACTAAAGA.CGTGGCATTG.GTGTATCGCA.GAGCAGGCG G.TACTACACAT.TCCGTAGCCG.CAGGTCACTT.CGGTC-3′.