Skip to main content
. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Regul Pept. 2013 Mar 27;184:96–103. doi: 10.1016/j.regpep.2013.03.028

Figure 4. Gαq-dependent activation of PI hydrolysis by melatonin.

Figure 4

Cultured gastric muscle cells labeled with myo-[3H]inositol and expressing Gαq minigene, Gαi minigene, or control vector were treated with melatonin (1 μM), cholecycstokinin (CCk, 1 nm) or cyclopentyladenosine (CPA, 1 μM) for 60 s. Total [3H]inositol phosphates were separated by ion-exchange chromatography. PI hydrolysis activity stimulated by melatonin or CCK was abolished in cells expressing Gαq minigene, but was not affected in cells expressing Gαi minigene. In contrast, PI hydrolysis activity stimulated by CPA was abolished in cells expressing Gαi minigene, but was not affected in cells expressing Gαq minigene. Results are expressed as total [3H]inositol phosphate formation in cpm/mg protein. Values are means ± SEM of four experiments. ** Significant inhibition from control response (P<0.01).