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. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: J Proteomics Bioinform. 2013 Mar 1;6(3):043–050. doi: 10.4172/jpb.1000260

Figure 1. Quantitative proteomic analysis of FFPE melanoma.

Figure 1

Melanocytes from benign FFPE skin biopsies (25 total) and melanoma cells from primary (12 total) and metastatic melanoma (24 total) FFPE biopsies were isolated by needle dissection. Protein extracts were resolved by SDS-PAGE, visualized by Coomassie staining, excised as 24 bands per lane, and subjected to in-gel trypsin digestion. Tryptic peptides were analyzed by LC-MS/MS and relative protein levels were determined by spectral counting [9].