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Optimized PEG purification of small DNA templates. 1 μg of plasmid DNA was digested with EcoRV and precipitated with 7% (lane 2 and 5), 8% (lane 3 and 6) and 9% of PEG (lane 4 and 7). DNA purified from both pellet (Lane 2,3,4) and supernatant (Lane 5,6,7) after PEG precipitation was run on 1% agarose gel and the ratio of small fragment (fragments) and backbone (vector) was determined. 0.5 μg of DNA directly purified after digestion was loaded in Lane 1 (Input).
