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. 2013 Aug;159(Pt 8):1725–1735. doi: 10.1099/mic.0.065532-0

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© 2013 SGM

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Fig. 1. — CS21-mediated ETEC adherence to epithelial cells. Adherence values correspond to the number of cell-associated bacteria after 3 h infection. E9034A is a wild-type ETEC strain, E9034AΔlngA is the CS21 isogenic mutant and E9034AΔlngA(pLngA) is the complemented CS21 mutant. IPEC-1 and IPEC-J2 are pig-derived primary intestinal cell lines grown in IPEC tissue culture media or IPEC media supplemented with 50 % TB. Absolute c.f.u. values are as millions of c.f.u. ml^–1. Error bars represent ±1 sd. Asterisks represent statistically significant differences in adherence to cells (P≤0.05) between wild-type ETEC (or completed CS21-mutant) and the CS21-mutant strain.