Table 2. Primers used in this study.
Multiplex PCR primers and assays for additional VFs: fyuA (Johnson & Stell, 2000), traT (Johnson & Stell, 2000), kpsMT II (Johnson & Stell, 2000), kpsMT III (Johnson & Stell, 2000), papAH (Johnson & Stell, 2000), sfa/focDE (Le Bouguenec et al., 1992), ibeA (Huang et al., 1995), hlyA (Johnson & Stell, 2000), cnf1 (Yamamoto et al., 1995), and clonal groups (Clermont et al., 2000) were performed as described. Primer groupings are listed in Methods.
| Primers (forward/reverse) | Target | Sequence (5′→3′) | Size | Reference |
| fimE MPX 1/fimE MPX2 | fimE | CTAACTGGAAAGGCGCTGAC/GAATATTTCGATGCCCGAGA | 225 | This study |
| fimB MPX 1/fimB MPX2 | fimB | GCCTCATGCTGCACGTAAT/CAATCGACAAATTTCACTCG | 79 | This study |
| fimX MPX 1/fimX MPX 2 | fimX | CCAGAGCATGTCCTTTCCTG/TTCCTCTGCTTAAGCCACAAC | 216 | This study |
| ipuA MPX 1/ipuA MPX 2 | ipuA | GCGATGTTTGCATGATTTTA/TTTTACCCGCAGCAGAAACT | 303 | This study |
| ipuB MPX 1/ipuB MPX 2 | ipuB | TGCGCAAATTTATTACTCATAGTG/TGTCTCGAGATTTTATTTCCTTGA | 334 | This study |
| hyxR MPX 1/hyxR MPX 2 | hyxR | TCGATGAGCGGAATGTTGTC/GGCTGCTCTATACGGGATGC | 89 | This study |
| hyxA MPX 1/hyxA MPX 2 | hyxA | GCATTTCCATCACCGTGAAA/GTGCGCAGTTTCTCAAAACG | 142 | This study |
| hyxB MPX 1/hyxB MPX 2 | hyxB | GGGTATCACCACCCAGCATT/CAGGATGCTGTCCGTCTGAG | 139 | This study |