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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Thromb Haemost. 2012 Nov 29;109(1):118–126. doi: 10.1160/TH12-04-0237

Figure 2. Knockdown of PP1c isoforms and overexpression of PP1cα in 293 αIIbβ3 cells.

Figure 2

(A) Lysate from the control siRNA and PP1cγ1 siRNA-treated cells were immunoblotted with PP1cγ1 PP1cα, PP1cβ and actin antibodies. (B) Lysate from control and PP1cβ siRNA-treated cells were immunoblotted with anti-PP1cβ, anti-PP1cα, anti-PP1cγ1 and actin antibodies. (C) PP1cα, PP1cβ, PP1cγ1 and actin expression in 293 αIIbβ3 cells treated with control and PP1cα siRNAs. (D) Immunoblotting with anti-PP1cα and HA antibodies in the control vector and HA-tagged PP1cα overexpressing cells. Knockdown and overexpression blots are representative of 2-3 experiments.