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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Thromb Haemost. 2012 Nov 29;109(1):118–126. doi: 10.1160/TH12-04-0237

Figure 4. Effect of depleting the PP1c isoforms on fibrin clot retraction.

Figure 4

(A) The decreased fibrin clot retraction of PP1c (α, β, γ1) depleted cells compared to the control siRNA treated cells was significant at *p=0.03, *p=0.01, *p=0.001 for 30, 60 and 120 minutes, respectively. The increased fibrin clot retraction of PP1cα depleted cells compared to the control siRNA treated cells was significant at ◆p=0.05, ◆p=0.04, p=0.07 for 30, 60 and 120 minutes, respectively. The decreased fibrin clot retraction of PP1cβ depleted cells compared to the control siRNA treated cells was significant at †p=04, †p=.0.03, †p=0.005 for 30, 60 and 120 minutes, respectively. Integrilin (5 μM) blocked the clot retraction of control siRNA cells at 120 minutes *p=0.003. n=3. (B) PP1c α, β and γ1 GST proteins separated on a SDS-PAGE gel were detected by Coomassie Blue staining. M is Molecular weight marker. (C). αIIb in the lysate from the 293 αIIbβ3 cells and platelets interact with PP1cα–GST, PP1cβ-GST, PP1cγ1–GST but not GST. Comparable levels of αIIb in lysate (input) was used for pull down studies. n=2-3. (D) 293 αIIbβ3 cell lysate was immunoprecipitated (IP) with IgG (control) or αIIb and immunoblotted (IB) with anti- PP1cα, PP1cβ, PP1cγ1 and αIIb. Lys is cell lysate. n=2.