Table II. Mass spectrometric analysis and identification of the O-linked glycans from wild type and Camnt1- Camnt2Δ mutant strains.
Strain/Peak | Composition Man-Mannitol |
Linkage composition |
ES-MS [M + Na]+ | |||
---|---|---|---|---|---|---|
t-Man | 2-O-Man | 2-O-Mannitol | Mannitol | |||
CAF2–1/1 (Man1) | 0–1 | 0 | 0 | 0 | 1 | nda |
CAF2–1/2 (Man2) | 1–1.3 | 1 | 0 | 0.7 | 0 493 | Da |
CAF2–1/3 (Man3) | 3.4–1 | 1 | 1 | 1 | 0 | 697 Da |
CAF2–1/4 (Man5) | 4.5–1 | 1 | 2.7 | 0.8 | 0 | 1105 Da |
Camnt1-Camnt2Δ/1 (Man1) | 0–1 | 0 | 0 | 0 | 1 | nd |
Camnt1-Camnt2Δ/3 (Man3) | 3.5–1 | 0.5 | 1 | 0.7 | 0 | 697 Da |
Not determined.