(a) Confocal images of lung sections loaded with DHE, treated with 20-5,14-HEDE or vehicle, fixed with paraformaldehyde then probed with antibody to PECAM-1 are shown. The images show increased DHE fluorescence in sections treated with 20-5,14-HEDE versus vehicle control. (b) Averaged DHE fluorescence intensity from 20 confocal slices of vehicle-treated and 20 slices of 20-5,14-HEDE treated sections. These data were obtained from 4 separate experiments with 5 slices from each obtained around the peak of fluorescence.