Chemiluminescence of lung samples treated with 20-5,14-HEDGE or vehicle. Average weights of lung tissues were 19.5 ± 1 mg (vehicle) and 19.9 ± 1 mg (20-5,14-HEDGE; p=0.74, n.s.). Lung pieces were incubated with 20-5,14-HEDGE or vehicle for 15 minutes followed by addition of lucigenin. Luminescence was then detected every 10s for 5 minutes. “n” =4 samples for vehicle and 7 samples for 20-5,14-HEDGE. Bars represent standard error of the means. 20-5,14-HEDGE increased chemiluminescence over time above that of vehicle treated samples. “*” indicates increased over vehicle treated control samples at the same time period after addition of 20-5,14-HEDGE (p<0.05).