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. 2013 Aug 21;8(8):e70393. doi: 10.1371/journal.pone.0070393

Table 7. Functional annotation clustering of genes downregulated in stage I and then upregulated in stage II of lactogenesis.

Category Terms N Percent(%) P-Value
Cluster 1 Enrichment Score: 12.80
GOTERM_CC_FAT GO:0005576∼extracellular region 36 41.38 2.88E-17
UP_SEQ_FEATURE signal peptide 37 42.53 6.67E-15
SP_PIR_KEYWORDS signal 37 42.53 1.07E-14
SP_PIR_KEYWORDS Secreted 29 33.33 9.55E-14
SP_PIR_KEYWORDS disulfide bond 32 36.78 9.52E-13
UP_SEQ_FEATURE glycosylation site:N-linked (GlcNAc…) 34 39.08 3.16E-12
SP_PIR_KEYWORDS glycoprotein 35 40.23 6.54E-12
UP_SEQ_FEATURE disulfide bond 29 33.33 9.64E-11
Cluster 2 Enrichment Score: 5.50
GOTERM_CC_FAT GO:0044421 extracellular region part 20 22.99 8.79E-10
GOTERM_CC_FAT GO:0031012 extracellular matrix 11 12.64 1.96E-06
SP_PIR_KEYWORDS extracellular matrix 7 8.05 1.36E-04
GOTERM_CC_FAT GO:0005578 proteinaceous extracellular matrix 8 9.20 4.14E-04
Cluster 3 Enrichment Score: 5.46
INTERPRO IPR014716:Fibrinogen, alpha/beta/gamma chain, C-terminal globular, subdomain 1 6 6.90 2.87E-07
INTERPRO IPR002181:Fibrinogen, alpha/beta/gamma chain, C-terminal globular 6 6.90 7.05E-07
SMART SM00186:FBG 6 6.90 3.55E-06
UP_SEQ_FEATURE domain:Fibrinogen C-terminal 4 4.60 2.02E-04
Cluster 4 Enrichment Score: 3.68
GOTERM_BP_FAT GO:0002526 acute inflammatory response 7 8.05 5.29E-07
GOTERM_BP_FAT GO:0006954 inflammatory response 8 9.20 8.54E-06
GOTERM_BP_FAT GO:0002541 activation of plasma proteins involved in acute inflammatory response 5 5.75 8.59E-06
GOTERM_BP_FAT GO:0006956 complement activation 5 5.75 8.59E-06
GOTERM_BP_FAT GO:0006957 complement activation, alternative pathway 4 4.60 1.21E-05
SP_PIR_KEYWORDS complement alternate pathway 4 4.60 2.82E-05
GOTERM_BP_FAT GO:0006959 humoral immune response 5 5.75 3.19E-05
GOTERM_BP_FAT GO:0009611 response to wounding 9 10.34 3.22E-05
GOTERM_BP_FAT GO:0045087 innate immune response 6 6.90 7.05E-05
GOTERM_BP_FAT GO:0051605 protein maturation by peptide bond cleavage 5 5.75 1.48E-04
GOTERM_BP_FAT GO:0050778 positive regulation of immune response 6 6.90 2.14E-04
GOTERM_BP_FAT GO:0002253 activation of immune response 5 5.75 3.40E-04
SP_PIR_KEYWORDS innate immunity 5 5.75 3.93E-04
GOTERM_BP_FAT GO:0016485 protein processing 5 5.75 5.02E-04
KEGG_PATHWAY bta04610:Complement and coagulation cascades 5 5.75 5.36E-04
GOTERM_BP_FAT GO:0051604 protein maturation 5 5.75 6.23E-04
GOTERM_BP_FAT GO:0006952 defense response 8 9.20 0.00
GOTERM_BP_FAT GO:0048584 positive regulation of response to stimulus 6 6.90 0.00
GOTERM_BP_FAT GO:0002252 immune effector process 5 5.75 0.00
GOTERM_BP_FAT GO:0002684 positive regulation of immune system process 6 6.90 0.00
SP_PIR_KEYWORDS immune response 5 5.75 0.00
GOTERM_BP_FAT GO:0006955 immune response 7 8.05 0.02
GOTERM_BP_FAT GO:0006508 proteolysis 9 10.34 0.07
Cluster 5 Enrichment Score: 2.84
KEGG_PATHWAY bta04512:ECM-receptor interaction 7 8.05 2.30E-06
INTERPRO IPR013032:EGF-like region, conserved site 9 10.34 7.39E-06
INTERPRO IPR006210:EGF-like 7 8.05 1.20E-04
SMART SM00181:EGF 7 8.05 6.99E-04
INTERPRO IPR013111:EGF, extracellular 4 4.60 0.00
GOTERM_BP_FAT GO:0007155 cell adhesion 9 10.34 0.00
GOTERM_BP_FAT GO:0022610 biological adhesion 9 10.34 0.00
KEGG_PATHWAY bta04510:Focal adhesion 6 6.90 0.00
SP_PIR_KEYWORDS cell adhesion 6 6.90 0.00
GOTERM_BP_FAT GO:0007160 cell-matrix adhesion 3 3.45 0.03
GOTERM_BP_FAT GO:0031589 cell-substrate adhesion 3 3.45 0.03
INTERPRO IPR002035:von Willebrand factor, type A 3 3.45 0.04
SMART SM00327:VWA 3 3.45 0.08