. 2013 Aug 21;8(8):e72757. doi: 10.1371/journal.pone.0072757

Table 3. Analysed nutrient and amino acid composition of the amino acid imbalanced and balanced diets.

	Balanced diet	Imbalanced diet
Dry matter (DM; g kg⁻¹)	976	965
Crude protein (g kgDM⁻¹)	388	377
Crude fat (g kgDM⁻¹)	144	137
Starch (g kgDM⁻¹)	296	301
Ash (g kgDM⁻¹)	73	72
Digestible energy^* (kJ gDM⁻¹)	17.95	17.52
Amino acid composition (% crude protein)
Arginine	3.94	4.11
Histidine	1.80	1.88
Isoleucine	3.32	3.53
Leucine	6.03	6.37
*Lysine*	*5.13* ^†	*2.73*
*Methionine*	*2.35* ^†	*1.62*
Phenylalanine	4.23	4.46
Threonine	2.53	2.68
Tryptophan	0.77	0.85
Valine	3.58	3.82
Cysteine	1.52	1.64
Tyrosine	2.58	2.73
Glutamic acid	25.03	30.58

Digestible energy determined under normoxia treatment of the present study [31]

^†

For rainbow trout the estimated requirement for lysine, vary from 4.5 to 6.3% crude protein [32] and for methionine, it varies from 1.8 to 2.14% crude protein depending on level of cysteine [13], [32].