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. 2013 Aug 21;8(8):e68547. doi: 10.1371/journal.pone.0068547

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© 2013 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Grouped mice were sensitized to HRP or only treated with adjuvant SEB or CT. A–G, representative confocal images show the expression of Cldn2 (in green) on the epithelia. Panel C2 is an enlarged image spot in panel C (pointed by an arrow). The red color is the nucli that was stained with propidium iodide. H, the immune blots show the Cldn2 protein (22 kDa) in the mouse jejunal epithelia. The table below indicates the summarized integrated density of the immune blots. *, p<0.01, compared with the saline group. Each group consisted of 6 mice. Samples from each mouse were processed separately.