Table 1. Cldn primers and qRT-PCR results (%β-actin).
Cldns | Primers | NCBI# | Results | ||
Forward | Reverse | Mean | SD | ||
Cldn1 | ttttcccgatgacctttctg | agtttgcaggatctgggatg | NM_016674 | −2.8 | 0.6 |
Cldn2 | aaggtgctgctgagggtaga | ttgagcattcaaagcacagg | NM_016675 | 780.5* | 65.2 |
Cldn3 | gcacccaccaagatcctcta | agcctgtctgtcctcttcca | NM_009902 | −1.8 | 0.4 |
Cldn4 | acaggtcctgggaatctcct | cactgcatctgacctgtgct | NM_009903 | −2.6 | 0.5 |
Cldn5 | gctctcagagtccgttgacc | ctgccctttcaggttagcag | NM_013805 | −3.3 | 0.8 |
Cldn6 | tgagtccaagctccacctct | cttgggactgggacaggata | NM_018777 | 1.9 | 0.5 |
Cldn7 | agcatgttcctggattggtc | cctggacaggagcaagagag | NM_001193619 | −2.2 | 0.5 |
Cldn8 | tcccaaggcgtacagatttc | cactctccactgaggcatga | NM_018778 | −3.6 | 1.2 |
Cldn9 | taatgggtgaaggcttccag | gctcttggcctgaatcactc | NM_020293 | 18.2 | 6.8 |
Cldn10 | gtctgtgattaccgccacct | gccaagcaagcaattttagc | NM_001160098 | −2.9 | 1.1 |
Cldn11 | accacttcttggctgcctta | acccaagtcagcaatgttcc | NM_008770 | −7.7 | 2.9 |
Cldn12 | ttgaatgtgagacggtgcat | taaacatgaggcagcacagc | NM_001193659 | −6.5 | 2.6 |
Cldn13 | acttgctggagctcgacatt | ctcttgtttgctgacgacca | NM_020504 | −1.2 | 0.4 |
Cldn14 | aatgaggcaaagccagaaga | aggaagcctaggagctggac | NM_001165926 | −1.6 | 0.5 |
Cldn15 | ctctacttgggctggagtgc | caggccagagcttcctacac | NM_021719 | 21.3 | 7.2 |
Cldn16 | tttacgtcgaacgctcctct | tgaatagggcttcctcatgg | NM_053241 | −3.3 | 1.2 |
Cldn17 | gccaaggatgagaatggaaa | tccatctgagggaactttgg | NM_019753 | −4.2 | 1.6 |
Cldn18 | gagtgccgaggctacttcac | agatgccggagatgatgaac | NM_001194921 | 8.6 | 2.8 |
Cldn19 | tcctcttggcaggtctctgt | gtgcagcagagaaaggaacc | NM_153105 | −4.4 | 1.5 |
Cldn20 | gtgtctggggtgtctggagt | gtacaaatcccccaagcaga | NM_001101560 | −2.8 | 1.2 |
Cldn22 | agggtcttgatgtccctgtg | tggtctcatcccagaactcc | NM_029383 | 9.5 | 4.2 |
Cldn23 | ctccctgcttcagtctccag | ggtatctcgggaacaggaca | NM_027998 | −1.2 | 0.5 |
Cldn26 | ctcagtggatggcaaccttt | tcaggtgataagggctccac | NM_029070 | −3.3 | 1.4 |
Cldn27 | tccaacctagggagctgaga | tgggaaagggaacaaaactg | NM_001085535 | −5.8 | 2.3 |
, p<0.01, compared with mice treated with saline. The data represent 6 experiments.