Figure 2. Methylation of the FGA promoter reduces expression of a reporter gene in fibrinogen expressing cells.

(A) Linear constructs containing the differentially methylated FGA promoter (FGAP) and the firefly (ff) luciferase gene (luc). The differentially methylated promoter constructs were ligated to the luciferase gene and directly transfected without cloning steps. (B) Luciferase assays performed in fibrinogen expressing HuH7 cells and HEK293T control cells. Signals for the ligated fragments are normalized to the signal of the unligated luciferase gene. Bars show mean signals plus SEM, n=3. Statistical testing was performed using an unpaired t test (*P< .05, **P< .01, ***P< .001).