Figure 3. Effect of cyclosporin A (CsA) on type I interferon expression.

Wa rotavirus-infected HT-29 cells were treated with CsA at indicated doses at 12 h post-infection. Total cell lysates were collected at 24 h post-treatment. Total cellular RNA extracted from the total cell lysates was subjected to the real-time RT-PCR to quantify IFN-α (A) and IFN-β (B). IFN-α/IFN-β expression is expressed as mRNA levels relative (fold) to the control (uninfected HT-29 cells without CsA treatment, which is defined as 1). Data shown are expressed as mean ± standard deviation of triplicate cultures, and 3 independent experiments were carried out (*P<0.05, **P<0.01).