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PE/CA-PJ15 cells were treated with four shRNA plasmids against NNMT (pLKO. 1-330, 1-448, 1-164, 1-711), or with transfection reagent only (mock). (A) Real-Time PCR was used to analyze the amount of NNMT mRNA in transfected compared to mock cells. (B) Protein lysates, obtained from NNMT silenced and mock cells, were analyzed for NNMT expression by Western blot.
