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. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Nat Cell Biol. 2013 Jan 13;15(2):179–188. doi: 10.1038/ncb2661

FIGURE 6. Failure to restore Eps8 levels induces membrane blebbing and cell shape deformation after metaphase in HeLa cells.

FIGURE 6

(a) U2OS cells were treated with non-targeting siRNA (si nt) or siRNA targeting Eps8 (siEps8) for 72 hours. Cells were fixed and stained with anti-Eps8 antibodies and Hoechst. Scale bar = 10 μM.

(b) Still images of differential interference contrast (DIC) time lapses of mitotic HeLa cells. Cells were synchronized by a double thymidine block/release protocol and transfected with different combinations of si nt, si Eps8, and si Fbxw5 #3. 60 h after transfection, DIC time lapses were started and performed for 12-14 h (time interval 1.5 min). Representative examples of cells undergoing mitosis from control cells (left two time lapses) or Eps8-depleted cells (right two time lapses) are shown. ~40-60 cells were recorded per condition and classified as indicated. Scale bar = 10 μm