Skip to main content
. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Nat Cell Biol. 2013 Jan 13;15(2):179–188. doi: 10.1038/ncb2661

FIGURE 7. MEF Eps8 -/- cells exhibit a post-metaphase membrane blebbing phenotype that can be rescued by re-expression of Eps8 versions with actin capping activity.

FIGURE 7

(a) Still images of DIC time lapses of mitotic mouse embryo fibroblasts (MEFs). MEFs derived from Eps8 -/- mice5 and infected with either control pBABE-GFP vector (GFP) or pBABE-GFP-Eps8 WT, ΔCapping, or ΔBundling were seeded onto gelatine-coated coverslips at low confluency. The day after seeding, cells were subjected to time-lapse analysis (24 hours, time interval 2 minutes). 30 MEFs passaging through mitosis were recorded and classified per condition. Upper panels: fluorescence images taken immediately before the time-lapse. Lower panels: DIC images of the GFP-positive cells (white dashed line insets in upper panels) followed during mitosis. Bar = 10 μm.

(b) Quantification of the experiment in (a).

(c) Immunoblot analysis of expression levels of GFP-Eps8 versions in infected MEF Eps8 -/- cells. α-tubulin levels serve as loading control.