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. 2013 Aug 8;9(8):e1003679. doi: 10.1371/journal.pgen.1003679

Figure 6. The region of the germline with nuclear DSB-1 localization is extended in mutants with impaired crossover formation.

Figure 6

(A) Composite projection images of gonads from indicated genotypes showing immunofluorescence staining of DSB-1 and DAPI. Lines represent the start (left line) and end (right line) of the leptotene-zygotene-pachytene (LZP) region of the gonad, and the end of the zone of DSB-1 localization (middle line). (B) Quantification of the zone of DSB-1 localization, showing the percent, by length, of the LZP region positive for DSB-1 staining. Numbers in parentheses indicate the number of gonads quantified for each genotype. All genotypes showed significant differences from wild type, p<0.003 except for htp-1, for which p<0.05. Error bars indicate standard deviation. Genotypes are color-coded based on the category of meiotic defect that they cause (listed above the graph). (C) Quantification of the zone of DSB-1 localization in gamma-irradiated animals and unirradiated controls. Animals were irradiated (10 Gy) at 16 hours post L4, then dissected and fixed 8 hours later to measure the length of the zone of DSB-1 localization relative to the length of the LZP region. Numbers in parentheses indicate the number of gonad arms quantified. Error bars indicate standard deviation. **p = 0.0005, *p = 0.002.