Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 15;62(9):3120–3131. doi: 10.2337/db13-0214

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

PMC Copyright notice

FIG. 6. — IL-2 and RAPA effects on STAT5 phosphorylation. Prediabetic female NOD mice 12–16 weeks of age were injected with PBS, 1 mg/kg RAPA 16 and 2 h before analysis, 250,000 IU IL-2 2 h before the analysis, or the combined treatment (IL-2 + RAPA). Phosphorylation of STAT5 on T_reg cells (CD4⁺ Foxp3⁺ T cells) and T_eff cells (CD4⁺ Foxp3⁻ T cells) was determined by flow cytometry. Shown are the gating strategy to define the percentage of p-STAT5⁺ T_reg and T_eff cells (top) and the percentage of p-STAT5⁺ T_reg cells (bottom left) or T_eff cells (bottom right) in the DLN. Data are cumulative of three independent experiments, symbols represent individual mice, and horizontal lines represent the median. ***P < 0.001 (unpaired, two-tailed Student t test).