Figure 4.

Th2-specific cytokines increase upon OVA treatment of WT mice but not of K14-Act mice. Skin biopsies from WT and K14-Act mice, untreated or treated (vehicle-Ctl or OVA) were collected and used for RNA isolation and subsequent generation of cDNA. Real-time PCRs were performed using primers specific for (A) mIl13, (B) mIl4, and (C) mIfnγ. Ct values were normalized to expression levels of the hprt house-keeping gene. The experiment was realized in groups of 8–10 mice. Results are presented in a scatter graph and mean values ± SEM are shown. Statistically significant differences were calculated with the Mann–Whitney U-test (p < 0.05*, p < 0.01**, and p < 0.001***).