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GH activates STAT5 and ERK in breast cancer cells. A, T47D cells were treated for 30 minutes with GH, E2, or both. Phospho- and total STAT5 and ERK levels were assessed by Western blot. B, T47D cells were pretreated for 2 hours with vehicle (dimethylsulfoxide) or inhibitors for EGFR (AG1478, 15 μM) or JAK2 (AZD1480, 1 μM), followed by treatment with GH+E2 for 30 minutes.
