Figure 1.

The human FSHB promoter is not induced by androgen treatment in immortalized gonadotropes. The murine −1000 Fshb-luc, the human −1028 FSHB-luc, or the human −1028 FSHB-luc with a consensus HRE (+ HRE) was transiently transfected into LβT2 cells along with 200 ng of AR. A, After overnight starvation in serum-free media, cells were treated for 24 hours with 0.1% ethanol or 100nM R1881. B, The cells were treated for 24 hours with 0.1% ethanol or 100nM DHT. The results represent the mean ± SEM of 3 (A) or 4 (B) experiments performed in triplicate and are presented as fold androgen induction relative to vehicle. The different uppercase letters indicate that mFshb-luc transcription is significantly different from hFSHB-luc or hFSHB-luc + HRE using a 1-way ANOVA followed by Tukey's honestly significant difference post hoc test.