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. 2013 Jun 13;154(9):3016–3021. doi: 10.1210/en.2013-1294

Figure 2.

Figure 2.

The LHX3 homeodomain protein binds to a −221/−211 element in the human FSHB promoter. A, LβT2 nuclear extracts were incubated with a −360/−331 murine Fshb oligonucleotide (oligo) (lanes 1–4), a −213/−184 murine Fshb oligo (lanes 5–8), or a −229/−200 human FSHB oligo (lanes 9–12) and tested for complex formation in EMSA. Protein-DNA complexes are shown, with IgG control (lanes 2, 6, and 10), anti-FOXL2 antibody (lanes 3, 7, and 11), and anti-LHX3 antibody (lanes 4, 8, and 12). Antibody supershifts (ss) are indicated on the left and right. B, LβT2 nuclear extracts were incubated with a −229/−200 human FSHB oligo (lane 15) and tested for complex formation with competition from excess cold oligos containing single base pair mutations ranging from −222 to −209 (lanes 1–14). Abbreviations: WT, wild type; mut, mutant; mut comp, mutant competitor.