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. 2013 Jun 13;154(9):3016–3021. doi: 10.1210/en.2013-1294

Figure 3.

Figure 3.

LHX3 binding to the human FSHB promoter is reduced with the −211 G/T SNP. Nuclear extracts from murine LβT2 cells (A) or in vitro transcribed and translated (TnT) human LHX3 (B) were incubated with a −229/−200 human FSHB oligonucleotide (oligo) (lanes 1–3 and 5–14) or a −211 G/T mutant oligo (lane 4) and tested for complex formation in EMSA. LHX3-DNA complex is shown in lane 1, with the IgG control in lane 2 and the LHX3 supershift (ss) with an anti-LHX3 antibody in lane 3. Competition with 0.125 to 2 pmol of cold, wild-type −229/−200 FSHB oligo (WT comp) or the −211 G/T mutated oligo is shown in lanes 5 to 9 and 10 to 14, respectively. Abbreviations: WT comp, wild-type competitor; comp, competitor.