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FSHB transcription is reduced with the −211 G/T mutation in the human FSHB promoter. The wild-type (WT) human −1028 FSHB-luc and the −211 G/T mutation (mut) were transiently transfected into LβT2 cells, as indicated. The results represent the mean ± SEM of 6 experiments performed in triplicate and are presented as luc/β-gal. *, FSHB-luc transcription is significantly decreased with the −211 G/T mutation compared with the wild-type promoter using Student's t test.
