Figure 5.

Impacts of ArcA, Fnr and Crp on oxidases in S. oneidensis. (a) Promoter activities of the cco, cox and cyd operons in strains lacking ArcA, Fnr or Crp, respectively. Cells were prepared the same as in Figures 3b and 4. (b) Growth of the S. oneidensis wild-type and mutant strains under microaerobic conditions. All strains were grown in the defined medium with 1% O₂ in the gas atmosphere and growth was monitored at OD₆₀₀. Strains with indistinguishable phenotypes: WT=ΔarcA=Δfnr=ΔarcAΔfnr; Δcrp=ΔarcAΔcrp=ΔcrpΔfnr=ΔarcAΔcrpΔfnr. For clarity, only wild-type and single mutants ΔarcA, Δcrp and Δfnr are presented in the figure and error bars (s.d.<5% of presented data, n⩾3) are omitted.