Table 1. Strains and plasmids used in this studya.
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Host for regular cloning | Lab stock |
| WM3064 | Host for pir-dependent plasmids and donor strain for conjugation; ΔdapA | W Metcalf, UIUC |
| BL21(DE3) | Expression host for pTP247 | Lab stock |
| S. oneidensis | ||
| MR-1 | Wild type | Lab stock |
| HG0610 | petC deletion mutant derived from MR-1; ΔpetC | Gao et al., 2010a |
| HG0624 | crp deletion mutant derived from MR-1; Δcrp | Gao et al., 2010b |
| HG0624-2356 | crp and fnr double deletion mutant derived from MR-1; ΔcrpΔfnr | Gao et al., 2010b |
| HG2356 | fnr deletion mutant derived from MR-1; Δfnr | Gao et al., 2010b |
| HG2361 | ccoP deletion mutant derived from MR-1; ΔccoP | Gao et al., 2010a |
| HG2363 | ccoO deletion mutant derived from MR-1; ΔccoO | Gao et al., 2010a |
| HG2364 | ccoN deletion mutant derived from MR-1; ΔccoN | This study |
| HG2364-3285 | ΔccoNΔcydB | This study |
| HG2364-4606 | ΔccoNΔcoxB | This study |
| HG3285 | cydB deletion mutant derived from MR-1; ΔcydB | This study |
| HG3285-4606 | ΔcydBΔcoxB | This study |
| HG3988-0624 | ΔarcAΔcrp | Gao et al., 2008a |
| HG3988-2356 | ΔarcAΔfnr | Gao et al., 2008a |
| HG3988-0624-2356 | ΔarcAΔcrpΔfnr | Gao et al., 2008a |
| HG4606 | coxB deletion mutant derived from MR-1; ΔcoxB | Gao et al., 2010a |
| HG4607 | coxA deletion mutant derived from MR-1; ΔcoxA | This study |
| HGTRIOX | ΔccoNΔcydBΔcoxB | This study |
| Plasmids | ||
| pDS3.0 | Apr, Gmr, derivative from suicide vector pCVD442 | Lab stock |
| pHG101 | Promoterless broad-host Kmr vector | Wu et al., 2011 |
| pHG102 | pHG101 containing the S. oneidensis arcA promoter | Wu et al., 2011 |
| pTP247 | Gateway destination His-tag expression vector | Gao et al., 2008b |
| pTP247-Crp | pTP247 containing crp | This study |
| pTP327 | Apr, Tetr, lacZ reporter vector | Gao et al., 2010b |
| pTP327-Pcco-lacZ | pTP327 containing ∼400 bp upstream sequence of cco | This study |
| pTP327-Pcyd-lacZ | pTP327 containing ∼400 bp upstream sequence of cyd | This study |
| pTP327-Pcox-lacZ | pTP327 containing ∼400 bp upstream sequence of cox | This study |
a
plasmids containing mutational structures were constructed, as described in the text and not included in the table.