Table 1. Summary of sampling and gene discovery among both amplicon and shotgun-derived data sets.
| Target | Sponge | Method | No. of sequences | OTUsa | Coverage |
|---|---|---|---|---|---|
| Ketosynthase | Cymbastela concentrica | TEFAP | 324 | 7 | 0.99 |
| mWGS | 1 (17) | 1 | 0 | ||
| Scopalina sp. | TEFAP | 369 | 41 | 0.95 | |
| mWGS | 1 (8) | 1 | 0 | ||
| Cymbastela coralliophila | TEFAP | 15 | 11 | 0.53 | |
| mWGS | 5 (23) | 5 | 0 | ||
| Rhopaloeides odorabile | TEFAP | 350 | 17 | 0.97 | |
| mWGS | 107 (346) | 49 | 0.71 | ||
| Stylissa sp. | TEFAP | 39 | 16 | 0.74 | |
| mWGS | 0 (3) | 0 | N/A | ||
| Condensation | Cymbastela concentrica | TEFAP | 14 | 9 | 0.64 |
| mWGS | 4 (18) | 4 | 0 | ||
| Scopalina sp. | TEFAP | 3336 | 325 | 0.94 | |
| mWGS | 10 (51) | 4 | 0.70 | ||
| Cymbastela coralliophila | TEFAP | 1119 | 54 | 0.97 | |
| mWGS | 0 (18) | 0 | N/A |
Abbreviations: mWGS, metagenomic whole-genome shotgun sequencing; OUT, operational taxonomic units; TEFAP, tag-encoded FLX amplicon pyrosequencing.
a
OTUs were defined at 0.10 distance threshold. Numbers in brackets represent the total number of KS and C domain sequences obtained using PFAM identifiers.