Figure 5.

Phylogenetic relationships among intein splicing motifs from Bathycoccus (both metagenomic and PCR-based), the choanoflagellate S. rosetta, fungi, and the nearest outside group (inteins from bacterial and viral genes) are shown in this subtree derived from an analysis of 453 inteins (Supplementary Figure 5). Approximate maximum-likelihood inference was used on 51 amino-acid positions, representing conserved blocks A, B, F and G. NEB InBase identifiers precede species names. Node support is indicated where ⩾75% and substitutions per site (scale bar) are shown. PRP8 inteins are at site a unless noted otherwise.