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. 2013 Jul 25;109(4):1040–1050. doi: 10.1038/bjc.2013.420

Figure 3.

Figure 3

Pazopanib induces autophagic flux and ERK1/2 phosphorylation in BC cells. (A) Lysates from 5637 and J82 cells treated with 20 μM pazopanib and 50 nM bafilomycin A1, alone or in combination, for 48 h were separated on 14% or 10% SDS–PAGE and probed with anti-LC3 or anti-p62 and anti-GAPDH Abs, respectively. (B) Lysates from 5637 and J82 cells untreated or treated with 20 μM pazopanib at different times were separated on 12% SDS–PAGE and probed with anti-pERK or anti-ERK Abs. Densitometric analysis, expressed as the mean±s.d., was performed by comparing treated with untreated cells, *P<0.01. (C) Lysates from 5637 and J82 cells untreated, treated for 48 h with 20 μM pazopanib or pre-treated for 1 h with 50 μM PD98059 before the addition of pazopanib, were separated on 14% SDS–PAGE and probed with anti-LC3 Abs. Densitometric analysis, expressed as the mean±s.d., was performed by comparing PD9859+pazopanib- with pazopanib-treated cells and pazopanib-treated with untreated cells, *P<0.01.