Figure 4.

Sunitinib induces lysosomial-dependent necrosis of BC cells. (A) 5637 and J82 cells treated with 20 μM sunitinib for 24 h were stained with Annexin V-FITC and PI and analysed by FACS. Data are expressed as percentage of positive cells and represent one out of three separate experiments. (B) Cell growth of 5637 and J82 cells, treated for 24 h with vehicle, 20 μM sunitinib or pre-treated for 1 h with 50 nM bafilomycin A1 or with 100 μM chloroquine before the addition of sunitinib, was evaluated by MTT assay at 24 h after treatment. Data shown are the mean±s.d. of three independent experiments. Statistical analysis was performed by comparing sunitinib- with vehicle-treated cells and sunitinib plus bafilomycin A1- with sunitinib-treated cells, *P<0.01. (C) Cell viability was assessed in 5637 and J82 cells treated for 24 h with vehicle, 20 μM sunitinib or pre-treated for 1 h with 50 nM bafilomycin A1 before the addition of sunitinib by PI staining and FACS analysis. Data are one out of three separate experiments.