[Table/Fig-3]:

(Fluorescent In Situ Hybridization i.e., FISH) is showing triple color FISH (X centromere as green, SRY as red & Y centromere as yellow due to superimposed red with green) on a metaphase. Two green indicates 2 centromeres of X chromosome and one SRY (red) on Xpter. Absence of yellow (superimposed red & green) spot indicates no Y chromosome centromeres