FIG. 7.

H₂O₂ preconditioned CPCs increase endothelial cell density following I/R. After 28 days, hearts were incubated with isolectin and an antiluciferase antibody to determine the density of endothelial and implanted cells, respectively. Isolectin-positive cells are shown in red, firefly luciferase-positive cells are shown in green, and 4′,6-diamidino-2-phenylindole (DAPI) stained nuclei are shown in blue. Representative images of control CPC (A) and H₂O₂ (B) preconditioned CPC hearts were taken using confocal microscopy (40×objective). Sections from 100 μM H₂O₂ preconditioned CPC hearts exhibited a greater number of total endothelial cells (C) compared to untreated CPC I/R hearts. There were no significant differences in total Luciferase⁺ cells (D) or endothelial cells that stained for luciferase (E). Data are mean±SEM. Statistical significance was determined by One-Way ANOVA followed by the Tukey–Kramer Post hoc test. #P<0.01, n=4–8. Scale bar=10 μM.