FIG. 1.

(A) Composition of antibody cocktail. Table 1 lists the clone, isotype, source, and function of each of the antibodies (amine reactive dye is not an antibody) used in the cocktail. (B) Gating strategy for dendritic cells (DCs). The first step is to use a forward scatter (FSC)-A vs. side scatter (SSC)-A⁴⁴ plot to exclude the cellular debris. This is followed by using an FSH-H vs. an FSH-A plot to gate on singlet events. The third step is to exclude dead cells (the cells that stain bright for amine reactive dye). Finally, lin-1⁻ cells (DCs) are gated out and further categorized as myeloid dendritic cells (mDCs) or plasmacytoid dendritic cells (pDCs) on the basis of CD11c and CD123 staining.