FIG. 2.

Characterizing the expression of DC markers on fresh, frozen, and cultured peripheral blood mononuclear cells (PBMCs) in healthy donors. The analysis for one representative sample out of six healthy samples is shown. (A) From the gated mDC population, the expression of each phenotypic marker was analyzed by plotting histograms of fresh, cultured, and frozen cells of the same donor. The bars indicate the positive gates. (B) The same peaks described in (A) were created from the gated pDC population.