Skip to main content
. 2013 Apr 10;22(17):2402–2413. doi: 10.1089/scd.2012.0426

FIG. 3.

FIG. 3.

Expression of stem cell-associated and senescence-associated genes after small molecule treatment. (A) Flow cytometry analysis of STRO-1 and pluripotency-associated gene protein products after 5 days of small molecule treatments. Expression levels of STRO-1 and pluripotent genes after small molecule treatments of DPSCs at passages 3 and 10. (B) RT-PCR gel images of amplified target genes and the band intensities were quantitated as shown in bar charts on the right. (C) qPCR analysis showing relative levels of gene expression with or without small molecule treatments for 5 days. * Significantly different between treated and CTRL (ANOVA; P<0.05). (D) RT-PCR analysis of p16Ink4A expression after 5 days of small molecule treatment of DPSCs at passages 3 and 10. RT-PCR gel images of amplified target genes are shown in the top panel and the band intensities of DPSCs at P10 were quantitated as shown in the bottom graph. Data represent two independent experiments each performed in triplicate. RT-PCR, reverse transcription-polymerase chain reaction; qPCR, real time polymerase chain reaction.