Fig. 5.

Confirmation of transcriptional profiling results for the A909 and A909ΔliaR comparison. Ten micrograms of total RNA was prepared from A909, A909ΔliaR and the complemented strain A909ΔliaR+pLiaR and analysed by Northern blot. Probes were for the genes indicated: SAK_1439 (pili island PI-2b); SAK_0890 (penicillin-binding protein 2b); SAK_0658 (murN); SAK_2031 (spxA). In each case, expression was decreased in the A909ΔliaR strain as predicted by microarray analysis, and was restored with liaR expression from plasmid pKPN2liaR. DNA for recA (not shown to be regulated in the liaR microarray dataset) was used as control.