Table 3. Transcriptional profiling A909LiaR.

The number of genes whose expression levels were found to be significantly altered in A909ΔliaR versus wild-type GBS strain A909 is presented. Expression levels lower than 0.5 (−) and greater than 2.0 (+) were determined to be significant. Genes were classified into functional groups using the Sagalist server (http://genolist.pasteur.fr/SagaList/index.html) classification scheme (Moszer et al., 1995). The total number of genes per group is indicated, and groups in which the variation is significant by Fisher’s Exact test are indicated by the presence of the P-value. NS, not significant.

		Number of genes regulated in A909ΔLiaR
Functional classification category	Total genes in A909	Up-regulated	P-value	Down-regulated	P-value
1.1 Cell wall	81	2	ns	5	ns
1.2 Transport/binding proteins and lipoproteins	255	21	0.0031	2	<0.0001
1.3 Sensors (signal transduction)	20	1	ns	4	0.0038
1.4 Membrane bioenergetics	26	1	ns	3	ns
1.8 Cell surface proteins	55	5	ns	7	0.0469
2.1.1 Metabolism of carbohydrates	112	6	ns	6	ns
2.1.2 Main glycolytic pathways	13	0	ns	1	ns
2.2 Metabolism of amino acids	76	1	ns	8	ns
2.3 Metabolism of nucleotides/nucleic acids	68	1	ns	1	ns
2.4 Metabolism of lipids	37	8	0.0006	2	ns
2.5 Metabolism of coenzymes	36	3	ns	1	ns
2.6 Metabolism of phosphate	2	0	ns	0	ns
3.1 DNA replication	28	1	ns	0	ns
3.2 DNA restriction/modification and repair	26	0	ns	3	ns
3.3 DNA recombination	12	0	ns	2	0.0164
3.4 DNA packaging and segregation	17	0	ns	1	ns
3.5.2 Regulation	113	2	ns	9	ns
3.6 RNA modification	26	0	ns	0	ns
3.7.1 Ribosomal proteins	55	2	ns	0	ns
3.7.2 Aminoacyl-tRNA synthetases	22	0	ns	0	ns
3.7.3 Initiation	4	0	ns	0	ns
3.8 Protein modification	20	0	ns	5	0.0086
4.1 Adaptation to atypical conditions	20	0	ns	1	ns
4.2 Detoxification	14	1	ns	5	0.0024
4.3 Phage-related functions	17	0	ns	2	ns
4.4 Transposon and IS	46	2	ns	2	ns
4.5 Miscellaneous	15	2	ns	1	ns
5.0 Similar to unknown proteins	623	19	0.0063	46	ns
6.0 No similarity	226	10	ns	2	0.0003
Total	2065	88		119