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. 2013 Jul;159(Pt 7):1437–1446. doi: 10.1099/mic.0.066258-0

Fig. 4.

Fig. 4.

(a) ALDH zymogram: 1–6, Saccharomyces cerevisiae ALDH controls: 1, 0.1 U; 2, 0.25 U; 3, 0.5 U; 4, 0.75 U; 5, 1 U; 6, 3 U; 7, blank; 8, S. gordonii V2016wt. (b) ADH zymogram: 1–3, S. gordonii V2016wt, V2016ΔadhE and V288; 4 and 5, S. sanguinis S7 and Blackburn; 6 and 7, S. gordonii CHI and 110-3; 8–10, S. sanguinis 133-79, SK36 and ATCC 10556. (ALDH zymogram of these strains was negative; data not shown). (c) ALDH zymogram: 1–4 (produced only acetaldehyde from ethanol), S. salivarius 101-1; S. sanguinis 104-5; S. salivarius 109-2, and S. sanguinis 109-3; 5 and 6 (produced both acetaldehyde and acetic acid from ethanol), S. oralis 108 and S. mitis 110-5; 7–11 (produced only acetic acid from ethanol), S. salivarius 101-7, S. mitis 104-4, S. salivarius 107-2, 110-1 and 110-4. (d) ADH zymogram of the same 11 strains displayed in (c). Note: only S. salivarius 107-2 displayed an ALDH band, which is different from AdhE. S. oralis 108 did not show detectable ADH.