Figure 2.

Analysis of the magnitude and breadth of the T-cell response in subjects with acute self-limiting infection (A–C) and subjects with chronic evolution (D–F). Peripheral blood mononuclear cell (PBMC) samples were tested by interferon-γ enzyme-linked immunospot assay (ELISpot) at 3 representative time points after disease onset (0–1 month, A and D; 6 months, B and E); between 12 and 24 months (C and F) against 7 peptide pools corresponding to core, NS3 protease, NS3 helicase, NS4, NS5a, and NS5b (pool I and II) antigens. To simplify, only responses above the threshold are shown. For all remaining subjects, ELISpot responses were negative at all time points tested. Numbers represent spot-forming cells per10⁶ PBMCs. For each patient, IL28B genotype is reported. Abbreviations: NT, not tested; PBMC, peripheral blood mononuclear cell; SFC, spot-forming cell; SNP, single-nucleotide polymorphism.