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. Author manuscript; available in PMC: 2014 Jul 16.

Published in final edited form as: Anal Chem. 2013 Jul 2;85(14):6767–6774. doi: 10.1021/ac400832w

A) LC-MS base peak chromatogram of intact proteins extracted from a 12 µm section of mouse kidney and MALDI image acquired from an adjacent section showing the distribution of signals at m/z 9914 (blue) and m/z 12,169 (red). B) ETD spectrum of the [M+13H]¹³⁺ precursor ion (m/z 936.8) identified as prothymosin alpha (uniprot accession P26350).

A) LC-MS base peak chromatogram of intact proteins extracted from a 12 µm section of mouse kidney and MALDI image acquired from an adjacent section showing the distribution of signals at m/z 9914 (blue) and m/z 12,169 (red). B) ETD spectrum of the [M+13H]¹³⁺ precursor ion (m/z 936.8) identified as prothymosin alpha (uniprot accession P26350).