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. 2013 Mar 25;6(4):440–468. doi: 10.3390/ph6040440

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Tolerance for mismatches between an siRNA and its target. siRNA seed region (bases 2–8) [112,143] and splice site (bases 10–11) [127,132,144] are the least tolerant of mismatches because of their active role in silencing. The 1^st nucleotide [53,145], the nucleotides of the 3’overhang (bases 20–21) [146,147], and bases 17–19 [144,148] are the most tolerant of mismatches, because their ability to base pair is at least partially blocked by Ago2. Position and base specific mismatches are tolerated at positions 8–16 [141].