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. 2013 Jun 11;89(6):201–225. doi: 10.2183/pjab.89.201

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© 2013 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Proposed catalytic mechanisms of some enzymes with a catalytic carboxyl group(s). (a) RNase T₁. The mechanism is based on refs. 31 and 44. The first step is intramolecular transphosphorylation, and the second step is hydrolysis. G, guanine. (b) Porcine pepsin A. The mechanism is based on ref. 96. (c) Aspergilloglutamic peptidase. The mechanism is based on ref. 186, and includes two water molecules found in the crystal structure, but the possibility that the reaction proceeds without the second water molecule cannot be excluded. (d) Sedolisin. The mechanism is based on ref. 174. Only the acylation process is shown. (e) Hen egg-white lysozyme. The mechanism is based on ref. 205. NAM, N-acetylmuramic acid.