Table 5. ANOVA sheet of non-PEGylated nanoparticles bearing temozolomide on percent drug entrapment.
| Table 5A | ||||||
|---|---|---|---|---|---|---|
| Response―PDE ANOVA for Response Surface Quadratic Model Analysis of variance table [Partial sum of squares-Type III] | ||||||
| Source | Sum of squares | df | Mean square | F-Value | p-value Prob > F |
|
| Model |
10077.61 |
14 |
719.8295 |
81.00017 |
< 0.0001 |
significant |
| A-Drug |
3856.411 |
1 |
3856.411 |
433.9499 |
< 0.0001 |
|
| B-PLGA |
3202.892 |
1 |
3202.892 |
360.4115 |
< 0.0001 |
|
| C-PVA |
62.46827 |
1 |
62.46827 |
7.02936 |
0.01 |
|
| D-ST |
43.722 |
1 |
43.722 |
4.919901 |
0.0299 |
|
| AB |
797.5917 |
1 |
797.5917 |
89.75052 |
< 0.0001 |
|
| AC |
0.831136 |
1 |
0.831136 |
0.093525 |
0.7607 |
|
| AD |
0.0324 |
1 |
0.0324 |
0.003646 |
0.952 |
|
| BC |
0.315469 |
1 |
0.315469 |
0.035499 |
0.8511 |
|
| BD |
0.026678 |
1 |
0.026678 |
0.003002 |
0.9565 |
|
| CD |
0.225625 |
1 |
0.225625 |
0.025389 |
0.8739 |
|
| A^2 |
1116.205 |
1 |
1116.205 |
125.603 |
< 0.0001 |
|
| B^2 |
917.3058 |
1 |
917.3058 |
103.2216 |
< 0.0001 |
|
| C^2 |
11.23415 |
1 |
11.23415 |
1.264144 |
0.2649 |
|
| D^2 |
2.025956 |
1 |
2.025956 |
0.227974 |
0.6346 |
|
| Residual |
595.4132 |
67 |
8.886765 |
|
|
|
| Lack of Fit |
595.2674 |
66 |
9.019204 |
61.86011 |
0.1008 |
not significant |
| Pure Error |
0.1458 |
1 |
0.1458 |
|
|
|
| Cor Total | 10673.03 | 81 | ||||
| Table 5B | |||
|---|---|---|---|
| Standard deviation | 2.981068 | R-Squared | 0.944213 |
| Mean |
63.94866 |
Adj R-Squared |
0.932556 |
| C.V. % |
4.661658 |
Pred R-Squared |
0.917066 |
| PRESS | 885.1574 | Adeq Precision | 28.43542 |
| Table 5C | ||||||
|---|---|---|---|---|---|---|
| Factor | Coefficient |
Standard error | 95% CI low | 95% CI high | VIF | |
| estimate | Df | |||||
| Intercept |
74.458 |
1 |
0.942696 |
72.57637 |
76.33963 |
|
| A-Drug |
8.450741 |
1 |
0.405672 |
7.641016 |
9.260465 |
1 |
| B-PLGA |
7.701481 |
1 |
0.405672 |
6.891757 |
8.511206 |
1 |
| C-PVA |
1.075556 |
1 |
0.405672 |
0.265831 |
1.88528 |
1 |
| D-ST |
-0.89981 |
1 |
0.405672 |
-1.70954 |
-0.09009 |
1 |
| AB |
4.706944 |
1 |
0.496845 |
3.715238 |
5.69865 |
1 |
| AC |
0.151944 |
1 |
0.496845 |
-0.83976 |
1.14365 |
1 |
| AD |
-0.03 |
1 |
0.496845 |
-1.02171 |
0.961706 |
1 |
| BC |
-0.09361 |
1 |
0.496845 |
-1.08532 |
0.898095 |
1 |
| BD |
0.027222 |
1 |
0.496845 |
-0.96448 |
1.018928 |
1 |
| CD |
-0.07917 |
1 |
0.496845 |
-1.07087 |
0.912539 |
1 |
| A^2 |
-7.78674 |
1 |
0.694793 |
-9.17355 |
-6.39993 |
1.001626 |
| B^2 |
-7.05896 |
1 |
0.694793 |
-8.44578 |
-5.67215 |
1.001626 |
| C^2 |
-0.78119 |
1 |
0.694793 |
-2.168 |
0.605628 |
1.001626 |
| D^2 | -0.33174 | 1 | 0.694793 | -1.71855 | 1.055073 | 1.001626 |
Final equation in terms of coded factors: PDE = 74.45+ 8.45A + 7.70B + 1.07C – 0.89D + 4.70AB + 0.15AC - 0.03AD – 0.09BC + 0.02BD – 0.07CD – 7.78A2 – 7.04B2 – 0.78C2 – 0.33D2… Eqn. 1.