Table 7. ANOVA sheet of non-PEGylated nanoparticles bearing temozolomide on particle size.
| Table 7A | ||||||
|---|---|---|---|---|---|---|
| Response―PS ANOVA for Response Surface Quadratic Model Analysis of variance table [Partial sum of squares-Type III] | ||||||
| Source | Sum of squares | df | Mean square | F-value | p-value Prob > F |
|
| Model |
1086.671 |
14 |
77.61934 |
287.9431 |
< 0.0001 |
Significant |
| A-Drug |
715.4784 |
1 |
715.4784 |
2654.198 |
< 0.0001 |
|
| B-PLGA |
51.58756 |
1 |
51.58756 |
191.3735 |
< 0.0001 |
|
| C-PVA |
113.9414 |
1 |
113.9414 |
422.6863 |
< 0.0001 |
|
| D-ST |
171.842 |
1 |
171.842 |
637.4793 |
< 0.0001 |
|
| AB |
0.103469 |
1 |
0.103469 |
0.383839 |
0.5377 |
|
| AC |
0.297025 |
1 |
0.297025 |
1.101868 |
0.2976 |
|
| AD |
0.028336 |
1 |
0.028336 |
0.105118 |
0.7468 |
|
| BC |
0.238469 |
1 |
0.238469 |
0.884646 |
0.3503 |
|
| BD |
0.005625 |
1 |
0.005625 |
0.020867 |
0.8856 |
|
| CD |
0.092011 |
1 |
0.092011 |
0.341332 |
0.561 |
|
| A^2 |
28.44803 |
1 |
28.44803 |
105.5332 |
< 0.0001 |
|
| B^2 |
0.016815 |
1 |
0.016815 |
0.062377 |
0.8035 |
|
| C^2 |
4.484401 |
1 |
4.484401 |
16.6357 |
0.0001 |
|
| D^2 |
0.506602 |
1 |
0.506602 |
1.879333 |
0.175 |
|
| Residual |
18.06084 |
67 |
0.269565 |
|
|
|
| Lack of Fit |
12.14404 |
66 |
0.184001 |
0.031098 |
1 |
not significant |
| Pure Error |
5.9168 |
1 |
5.9168 |
|
|
|
| Cor Total | 1104.732 | 81 | ||||
| Table 7B | |||
|---|---|---|---|
| Standard deviation | 0.519196 | R-Squared | 0.983651 |
| Mean |
115.273 |
Adj R-Squared |
0.980235 |
| C.V. % |
0.450406 |
Pred R-Squared |
0.977501 |
| PRESS | 24.85564 | Adeq Precision | 70.73664 |
| Table 7C | ||||||
|---|---|---|---|---|---|---|
| Factor | Coefficient |
Standard error | 95% CI low | 95% CI high | VIF | |
| estimate | Df | |||||
| Intercept |
115.856 |
1 |
0.164184 |
115.5283 |
116.1837 |
|
| A-Drug |
3.64 |
1 |
0.070654 |
3.498975 |
3.781025 |
1 |
| B-PLGA |
0.977407 |
1 |
0.070654 |
0.836382 |
1.118433 |
1 |
| C-PVA |
1.452593 |
1 |
0.070654 |
1.311567 |
1.593618 |
1 |
| D-ST |
-1.78389 |
1 |
0.070654 |
-1.92491 |
-1.64286 |
1 |
| AB |
-0.05361 |
1 |
0.086533 |
-0.22633 |
0.119109 |
1 |
| AC |
0.090833 |
1 |
0.086533 |
-0.08189 |
0.263553 |
1 |
| AD |
0.028056 |
1 |
0.086533 |
-0.14466 |
0.200776 |
1 |
| BC |
0.081389 |
1 |
0.086533 |
-0.09133 |
0.254109 |
1 |
| BD |
-0.0125 |
1 |
0.086533 |
-0.18522 |
0.16022 |
1 |
| CD |
0.050556 |
1 |
0.086533 |
-0.12216 |
0.223276 |
1 |
| A^2 |
-1.24311 |
1 |
0.121008 |
-1.48464 |
-1.00158 |
1.001626 |
| B^2 |
0.030222 |
1 |
0.121008 |
-0.21131 |
0.271756 |
1.001626 |
| C^2 |
0.493556 |
1 |
0.121008 |
0.252022 |
0.735089 |
1.001626 |
| D^2 | -0.16589 | 1 | 0.121008 | -0.40742 | 0.075645 | 1.001626 |
Final Equation in terms of coded factors: PS = 115.85+ 3.64A + 0.97B + 1.45C – 1.78D – 0.05AB + 0.09AC + 0.03AD – 0.08BC – 0.01BD – 0.05CD – 1.24A2 – 0.03B2 – 0.49C2 – 0.16D2…. Eqn. 2.