Table 8. ANOVA sheet for PEGylated nanoparticles bearing temozolomide on particle size.
| Table 8A | ||||||
|---|---|---|---|---|---|---|
| Source | Sum of squares | df | Mean square | F-value | p-value Prob > F |
|
| Model |
749.4104 |
14 |
53.52932 |
128.02 |
< 0.0001 |
Significant |
| A-Drug |
471.6476 |
1 |
471.6476 |
1127.986 |
< 0.0001 |
|
| B-PLGA |
101.9563 |
1 |
101.9563 |
243.8373 |
< 0.0001 |
|
| C-PVA |
67.15645 |
1 |
67.15645 |
160.6104 |
< 0.0001 |
|
| D-ST |
57.57736 |
1 |
57.57736 |
137.7012 |
< 0.0001 |
|
| AB |
0.005625 |
1 |
0.005625 |
0.013453 |
0.908 |
|
| AC |
0.1444 |
1 |
0.1444 |
0.345345 |
0.5587 |
|
| AD |
0.001344 |
1 |
0.001344 |
0.003215 |
0.9549 |
|
| BC |
0.056803 |
1 |
0.056803 |
0.135849 |
0.7136 |
|
| BD |
0.595469 |
1 |
0.595469 |
1.424116 |
0.2369 |
|
| CD |
0.0441 |
1 |
0.0441 |
0.105469 |
0.7464 |
|
| A^2 |
42.29364 |
1 |
42.29364 |
101.1489 |
< 0.0001 |
|
| B^2 |
0.43066 |
1 |
0.43066 |
1.029961 |
0.3138 |
|
| C^2 |
8.163949 |
1 |
8.163949 |
19.52479 |
< 0.0001 |
|
| D^2 |
0.00791 |
1 |
0.00791 |
0.018917 |
0.891 |
|
| Residual |
28.01488 |
67 |
0.418133 |
|
|
|
| Lack of Fit |
23.24083 |
66 |
0.352134 |
0.07376 |
0.9995 |
not significant |
| Pure Error |
4.77405 |
1 |
4.77405 |
|
|
|
| Cor Total | 777.4253 | 81 | ||||
| Table 8B | |||
|---|---|---|---|
| Standard deviation | 0.646632 | R-Squared | 0.963965 |
| Mean |
118.9363 |
Adj R-Squared |
0.956435 |
| C.V. % |
0.543679 |
Pred R-Squared |
0.949605 |
| PRESS | 39.1785 | Adeq Precision | 46.84067 |
| Table 8C | ||||||
|---|---|---|---|---|---|---|
| Factor | Coefficient |
Standard error | 95% CI low | 95% CI high | VIF | |
| Estimate | Df | |||||
| Intercept |
119.6103 |
1 |
0.204483 |
119.2022 |
120.0185 |
|
| A-Drug |
2.95537 |
1 |
0.087995 |
2.779731 |
3.13101 |
1 |
| B-PLGA |
1.374074 |
1 |
0.087995 |
1.198434 |
1.549714 |
1 |
| C-PVA |
1.115185 |
1 |
0.087995 |
0.939546 |
1.290825 |
1 |
| D-ST |
-1.03259 |
1 |
0.087995 |
-1.20823 |
-0.85695 |
1 |
| AB |
0.0125 |
1 |
0.107772 |
-0.20261 |
0.227614 |
1 |
| AC |
-0.06333 |
1 |
0.107772 |
-0.27845 |
0.15178 |
1 |
| AD |
0.006111 |
1 |
0.107772 |
-0.209 |
0.221225 |
1 |
| BC |
0.039722 |
1 |
0.107772 |
-0.17539 |
0.254836 |
1 |
| BD |
0.128611 |
1 |
0.107772 |
-0.0865 |
0.343725 |
1 |
| CD |
-0.035 |
1 |
0.107772 |
-0.25011 |
0.180114 |
1 |
| A^2 |
-1.51573 |
1 |
0.15071 |
-1.81655 |
-1.21491 |
1.001626 |
| B^2 |
-0.15295 |
1 |
0.15071 |
-0.45377 |
0.147867 |
1.001626 |
| C^2 |
0.665938 |
1 |
0.15071 |
0.365121 |
0.966756 |
1.001626 |
| D^2 | -0.02073 | 1 | 0.15071 | -0.32155 | 0.280089 | 1.001626 |
Final equation in terms of coded factors: PS = 119.61+ 2.95A + 1.37B + 1.11C – 1.03D + 0.01AB - 0.06AC + 0.01AD + 0.03BC + 0.02BD – 0.03CD – 1.51A2 – 0.01B2 + 0.66C2 – 0.02D2…. Eqn. 4.